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phospho-perk (thr980) antibody  (MedChemExpress)


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    Structured Review

    MedChemExpress phospho-perk (thr980) antibody
    Phospho Perk (Thr980) Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+perk/Phospho-PERK+(Thr980)+Antibody/custom%40hy-p81190%4042692205
    Average 94 stars, based on 1 article reviews
    phospho-perk (thr980) antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Unfolded protein response-activated NLRP3 inflammasome contributes to pyroptotic and apoptotic podocyte injury in diabetic kidney disease via the CHOP-TXNIP axis.
    Article Snippet: .. Group 1: normal glucose (NG) = 5 mM D-glucose; mannitol (MA) = 5 mM D-glucose+25 mM mannitol; high glucose (HG) = 30 mM D-glucose, continuous stimulation for 48 h. Group 2: Thapsigargin (TG; ER stress inducer; MCE, USA) concentration gradient (0, 50, 100, 200, and 300 nM), continuous stimulation for 24 h. Group 3: HG + GSK2656157 (GSK; PERK phosphorylation inhibitor; MCE, USA) concentration gradient (0, 1, 10, 20, and 40 μM), continuous stimulation for 24 h. Group 4: HG + 4μ8C (IRE1α inhibitor; MCE, USA) concentration gradient (0, 1, 10, 20, and 40 μM), continuous stimulation for 24 h. Group 5: NG; HG; HG + GSK (20 μM; 24 h). ..

    Phospho-proteomics:

    Article Title: Unfolded protein response-activated NLRP3 inflammasome contributes to pyroptotic and apoptotic podocyte injury in diabetic kidney disease via the CHOP-TXNIP axis.
    Article Snippet: .. Group 1: normal glucose (NG) = 5 mM D-glucose; mannitol (MA) = 5 mM D-glucose+25 mM mannitol; high glucose (HG) = 30 mM D-glucose, continuous stimulation for 48 h. Group 2: Thapsigargin (TG; ER stress inducer; MCE, USA) concentration gradient (0, 50, 100, 200, and 300 nM), continuous stimulation for 24 h. Group 3: HG + GSK2656157 (GSK; PERK phosphorylation inhibitor; MCE, USA) concentration gradient (0, 1, 10, 20, and 40 μM), continuous stimulation for 24 h. Group 4: HG + 4μ8C (IRE1α inhibitor; MCE, USA) concentration gradient (0, 1, 10, 20, and 40 μM), continuous stimulation for 24 h. Group 5: NG; HG; HG + GSK (20 μM; 24 h). ..

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Incubation:

    Article Title: WFS1-related isolated diabetes induced by a WFS1 missense mutation: focus on the isolated diabetes phenotype.
    Article Snippet: The denatured protein was separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to polyvinylidene fluoride (PVDF) membranes with a pore size of 0.45 μm (Millipore). .. Membranes were subsequently blocked with 5% bovine serum albumin, and then probed overnight at 4°C with primary anti-WFS1 (26995-1-AP, Proteintech 1:1,000), anti-IRE1-α (HY-P80828, MCE; 1:1,000), anti-pIRE1-α (HY-P86330; MCE; 1:1000) anti-PERK (HY-P80781, MCE; 1:1,000), anti-pPERK (HY-P81190; MCE; 1:1000); anti-ATF4(HY-P80486, MCE; 1:1,000); and anti-actin (66009-1-Ig, Proteintech; 1:2,0000) antibodies, followed by 1.5 h incubation at room temperature with secondary horseradish peroxidase–conjugated antibody (goat anti-rabbit, 7074P2, CST; 1:3,000 or goat anti-mouse, A0216, Beyotime; 1:1,000). .. Protein bands were detected by ChemiDoc XRS+ Chemiluminescence imaging system (BioRad). β-actin served as a loading control.

    Article Title: WFS1-related isolated diabetes induced by a WFS1 missense mutation: focus on the isolated diabetes phenotype
    Article Snippet: The denatured protein was separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to polyvinylidene fluoride (PVDF) membranes with a pore size of 0.45 μm (Millipore). .. Membranes were subsequently blocked with 5% bovine serum albumin, and then probed overnight at 4 °C with primary anti-WFS1 (26995-1-AP, Proteintech 1:1,000), anti-IRE1-α (HY- P80828 , MCE; 1:1,000), anti-pIRE1-α (HY- P86330 ; MCE; 1:1000) anti-PERK (HY- P80781 , MCE; 1:1,000), anti-pPERK (HY- P81190 ; MCE; 1:1000); anti-ATF4(HY- P80486 , MCE; 1:1,000); and anti-actin (66009-1-Ig, Proteintech; 1:2,0000) antibodies, followed by 1.5 h incubation at room temperature with secondary horseradish peroxidase–conjugated antibody (goat anti-rabbit, 7074P2, CST; 1:3,000 or goat anti-mouse, A0216, Beyotime; 1:1,000). .. Protein bands were detected by ChemiDoc XRS+ Chemiluminescence imaging system (BioRad). β-actin served as a loading control.

    Inhibition:

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Positive Control:

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Selection:

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Stable Transfection:

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Clinical Proteomics:

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Membrane:

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Infection:

    Article Title: PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.102852 14 of 22 Compound Supplier Catalogue number CAS number Used for Oligomycin A SelleckChem S1478 579- 13- 5 Complex V inhibition Bedaquiline Aladdin B413184 845533- 86- 0 Complex V inhibition Antimycin A Abcam AB141904 1397- 94- 0 Complex III inhibition Rotenone Solarbio R8130 83- 79- 4 Complex I inhibition 2- Deoxy- D- glucose Macklin D807272 154- 17- 6 Glycolysis inhibition CCCP Solarbio C6700 555- 60- 2 ETC uncoupler FCCP TargetMol T6834 370- 86- 5 ETC uncoupler Rosuvastatin Solarbio IR0150 147098- 20- 2 Inhibitor of cholesterol synthesis Mdivi- 1 Beyotime SC8028 338967- 87- 6 Inhibitor of mitochondrial division PD 0332991 isethionate - Palbociclib Sigma- Aldrich PZ0199 827022- 33- 3 Cdk4/6 inhibitor used as positive control for MSCETSA Tunicamycin Cell Signaling Technology 12819s 11089- 65- 9 Induces ER stress and eIF2a phosphorylation via PERK kinase Puromycin BBI- Life Sciences A610593- 0026 58- 58- 2 Used for puromycin selection for stable cell lines BAM15 MedChemExpress HY- 110284 210302- 17- 3 Electron transport chain uncoupler which does not depolarize plasma membrane DFOM Deferoxamine Sigma- Aldrich D9533 138- 14- 7 Chelates iron and induces ISR via HRI kinase Poly(I:C) MedChemExpress HY- 107202 24939- 03- 5 Mimics viral infection and activates ISR via PKR kinase Sodium (meta)arsenite Sigma- Aldrich S7400 7784- 46- 5 Induces oxidative stress and induces ISR via HRI kinase Trametinib MedChemExpress HY- 10999 871700- 17- 3 MEK inhibitor used to test if PEBP1mediated effect on ISR is related to PEBP1 function as a Raf kinase inhibitor Phorbol- 12- myristate- 13- acetate (PMA) Rhawn R038872 16561- 29- 8 Induction of PEBP1 phosphorylation at Ser153 Cheng, Meliala et al. eLife 2024;13:RP102852. .. DOI: https://doi.org/10.7554/eLife.102852 15 of 22 Compound Supplier Catalogue number CAS number Used for Sephin- 1 Sigma- Aldrich SML1356 951441- 04- 6 Inhibitor of GADD34 (PPP1R15A), the stress- inducible eIF2α phosphatase subunit Raphin- 1 Aladdin R287653 2022961- 17- 5 Inhibitor of CREP (PPP1R15B), the constitutively expressed eIF2α phosphatase subunit

    Ubiquitin Proteomics:

    Article Title: Correction for Hou et al., Therapeutic restoration of mitochondria–endoplasmic reticulum cross talk for osteoarthritis
    Article Snippet: .. Antibodies Company Catalog # COLII Abcam Ab188570 ACAN ABclonal A11691 MMP13 Proteintech 18165-1-AP ADAMTS5 ABclonal A2836 MFN1 ABclonal A21293 MFN2 ABclonal A19678 OPA1 ABclonal A9833 DRP1 ABclonal A21968 FIS1 ABclonal A5821 SIRT3 Santa-curz sc-365175 USP47 ABclonal A15461 USP47 Santa-curz sc-100633 IRE1 Abcam Ab37073 IRE1 (phospho S724) Abcam Ab48187 eIF2α Cell Signaling Technology 9722S eIF2α (phospho Ser51) Cell Signaling Technology 9721S PERK MCE HY-P80781 PERK (phospho Thr980) MCE HY-P80845 SERCA2 Santa-curz sc-376235 Calnexin Santa-curz sc-23954 Pan Acetyl-Lysine ABclonal A2391 25 P16 ABclonal A23882 P21 ABclonal A22460-PM P53 ABclonal A0263 HA-Tag Abcam ab184643 DDDK-Tag ABclonal AE092 β-actin Servicebio GB11001-100 CD63 Cell Signaling Technology 52090T TSG101 Abcam ab125011 Ubiquitin Abcam ab134953 Goat Anti-Mouse IgG (H+L) HRP Affinity Biosciences S0002 Goat Anti-Rabbit IgG (H+L) HRP Affinity Biosciences S0001 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) Abcam ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) Abcam ab150115 IgG ABclonal AC005 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) Abcam ab150079 Supplementary Table 4. ..



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